Tea Polysaccharides Help Form Chitosan Nanoparticles That Suppress Rice Pathogens In Vitro and on Detached Leaves

Plant Health & Interactions

A 2024 Scientific Reports study used tea polysaccharides extracted from commercial black tea to form chitosan nanoparticles (CTNp) and tested them against major rice pathogens.

Approximately 119-nm chitosan nanoparticles

Dynamic light scattering measured an average diameter of 118.7 nm and a zeta potential of +33.1 mV.

Strong in-vitro antimicrobial activity

Antimicrobial activity of tea-polysaccharide chitosan nanoparticles against rice pathogens

CTNp suppressed more than 90% of Pyricularia grisea hyphal growth and spore germination and inhibited Xanthomonas oryzae, outperforming bulk chitosan or tea polysaccharide alone under the tested conditions.

Detached leaves—not a field crop trial

The rice disease assay used leaves removed from 30-day-old plants, treated with CTNp and then inoculated with pathogens. Disease symptoms were strongly suppressed, but the study did not measure field disease control, crop yield or long-term plant performance. The authors explicitly call for field evaluation.

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Reference

  • Sathiyabama M et al. Green synthesis of chitosan nanoparticles using tea extract and its antimicrobial activity against economically important phytopathogens of rice. Scientific Reports. 2024;14:7381. https://doi.org/10.1038/s41598-024-58066-y

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